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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1966 1
1975 2
1981 1
1983 1
1989 1
1995 1
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2007 1
2009 1
2010 2
2011 2
2013 2
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2016 1
2018 2
2020 1
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2022 3
2023 1
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26 results

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Page 1
Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates.
Sabeti Azad M, Cardoso Batista A, Faulon JL, Beisel CL, Bonnet J, Kushwaha M. Sabeti Azad M, et al. J Vis Exp. 2022 Aug 9;(186). doi: 10.3791/64236. J Vis Exp. 2022. PMID: 36036615
Linear DNA can be rapidly and easily amplified by PCR to obtain high concentrations of the template, avoiding potential in vivo expression toxicity. ...A detailed protocol for an alternative strategy is presented here for using linear DNA templates for CFPS. …
Linear DNA can be rapidly and easily amplified by PCR to obtain high concentrations of the template, avoiding potential in viv …
Implementation and evaluation of simultaneous video-electroencephalography and functional magnetic resonance imaging.
Chaudhary UJ, Kokkinos V, Carmichael DW, Rodionov R, Gasston D, Duncan JS, Lemieux L. Chaudhary UJ, et al. Magn Reson Imaging. 2010 Oct;28(8):1192-9. doi: 10.1016/j.mri.2010.01.001. Epub 2010 Mar 16. Magn Reson Imaging. 2010. PMID: 20233646
EEG was recorded using a commercial MR-compatible 64-channel cap and amplifiers. Video recording was performed using a two-camera custom-made system with EEG synchronization. An in-house script was used to calculate signal to fluctuation noise ratio (SFNR) fr …
EEG was recorded using a commercial MR-compatible 64-channel cap and amplifiers. Video recording was performed using a …
Development of Leishmania Species Strains with Constitutive Expression of eGFP.
Carrasco J, Chang JH, Pineda L, Quintero I, Giovani R, Spadafora C, Lleonart R, Restrepo CM. Carrasco J, et al. J Vis Exp. 2023 Apr 21;(194). doi: 10.3791/64939. J Vis Exp. 2023. PMID: 37154542
The gene encoding eGFP was obtained from a commercial vector and amplified by polymerase chain reaction (PCR) to enrich it and add restriction sites for the BglII and KpnI enzymes. ...The expression vector with the cloned gene was propagated in E. coli, purified, and the p …
The gene encoding eGFP was obtained from a commercial vector and amplified by polymerase chain reaction (PCR) to enrich it and add re …
Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems.
Dopp JL, Reuel NF. Dopp JL, et al. J Vis Exp. 2021 Jun 14;(172):10.3791/62728. doi: 10.3791/62728. J Vis Exp. 2021. PMID: 34180897 Free PMC article.
After receiving DNA from a vendor, the gene fragment is PCR-amplified, cut, circularized, and cryo-banked. A small amount of the banked DNA is then diluted and amplified significantly (up to 10(6)x) using isothermal rolling circle amplification (RCA). ...
After receiving DNA from a vendor, the gene fragment is PCR-amplified, cut, circularized, and cryo-banked. A small amount of the bank …
Potential impacts from simulated vessel noise and sonar on commercially important invertebrates.
Hudson DM, Krumholz JS, Pochtar DL, Dickenson NC, Dossot G, Phillips G, Baker EP, Moll TE. Hudson DM, et al. PeerJ. 2022 Jan 26;10:e12841. doi: 10.7717/peerj.12841. eCollection 2022. PeerJ. 2022. PMID: 35127295 Free PMC article.
Juvenile and sub-adult blue crabs (Callinectes sapidus) and American lobsters (Homarus americanus) were exposed to simulated low-frequency vessel noise (a signal was low-pass filtered below 1 kHz to ensure low-frequency content only) and mid-frequency sonar (a 1-s 1.67 kHz contin …
Juvenile and sub-adult blue crabs (Callinectes sapidus) and American lobsters (Homarus americanus) were exposed to simulated low-frequency v …
Transcriptome analysis of single cells.
Morris J, Singh JM, Eberwine JH. Morris J, et al. J Vis Exp. 2011 Apr 25;(50):2634. doi: 10.3791/2634. J Vis Exp. 2011. PMID: 21540826 Free PMC article.
This method was developed previously by our lab as an alternative to other gene expression analysis techniques such as reverse-transcription or real-time polymerase chain reaction (PCR). This technique provides for linear amplification of the polyadenylated RNA beginning w …
This method was developed previously by our lab as an alternative to other gene expression analysis techniques such as reverse-transcription …
Amplitude-Based Filtering for Video Magnification in Presence of Large Motion.
Wu X, Yang X, Jin J, Yang Z. Wu X, et al. Sensors (Basel). 2018 Jul 17;18(7):2312. doi: 10.3390/s18072312. Sensors (Basel). 2018. PMID: 30018210 Free PMC article.
Video magnification reveals important and informative subtle variations in the world. ...An advantage of this algorithm is that it can handle large motions, whether they are linear or nonlinear. Our experimental results show that the proposed method can amplify …
Video magnification reveals important and informative subtle variations in the world. ...An advantage of this algorithm is that it ca …
Revealing sub-voxel motions of brain tissue using phase-based amplified MRI (aMRI).
Terem I, Ni WW, Goubran M, Rahimi MS, Zaharchuk G, Yeom KW, Moseley ME, Kurt M, Holdsworth SJ. Terem I, et al. Magn Reson Med. 2018 Dec;80(6):2549-2559. doi: 10.1002/mrm.27236. Epub 2018 May 30. Magn Reson Med. 2018. PMID: 29845645 Free PMC article.
The original aMRI approach used a video-processing algorithm, Eulerian video magnification (EVM), to amplify cardio-ballistic motion in retrospectively cardiac-gated MRI data. ...RESULTS: Phantom simulations showed that phase-based aMRI has a linear de …
The original aMRI approach used a video-processing algorithm, Eulerian video magnification (EVM), to amplify cardio-bal …
Improving gender-affirming care in genetic counseling: Using educational tools that amplify transgender and/or gender non-binary community voices.
Huser N, Hulswit BB, Koeller DR, Yashar BM. Huser N, et al. J Genet Couns. 2022 Oct;31(5):1102-1112. doi: 10.1002/jgc4.1581. Epub 2022 Apr 23. J Genet Couns. 2022. PMID: 35460542 Free PMC article.
Video testimonials featuring TGNB individuals complemented learning within each module. ...Linear regression did not identify an impact of practice specialty on participants' knowledge gains or self-efficacy. ...
Video testimonials featuring TGNB individuals complemented learning within each module. ...Linear regression did not identify …
Heterologous expression of azurin from Pseudomonas aeruginosa in the yeast Pichia pastoris.
Unver Y, Sensoy Gun B, Acar M, Yildiz S. Unver Y, et al. Prep Biochem Biotechnol. 2021;51(7):723-730. doi: 10.1080/10826068.2020.1855444. Epub 2020 Dec 21. Prep Biochem Biotechnol. 2021. PMID: 33346686
In this study, the production of azurin under the control of the alcohol oxidase promoter which is frequently used in the Pichia pastoris expression system was performed. The azurin gene amplified from Pseudomonas aeruginosa genomic DNA and inserted into the pPICZalphaA wa …
In this study, the production of azurin under the control of the alcohol oxidase promoter which is frequently used in the Pichia pastoris ex …
26 results